Enter one data set into column A, and use columns B, C. If you prefer to enter concentrations, rather than the logarithm of concentrations, use Prism to transform the X values to logs.įrom the data table, click Analyze, choose nonlinear regression, choose the panel of equations "Dose-response curves - Stimulation" and then choose the equation " log(Agonist) vs. Ĭonsider constraining the parameter HillSlope to its standard values of 1.0. This is especially useful if you don't have many data points, and therefore cannot fit the slope very well. If you have subtracted off any basal response, consider constraining Bottom to a constant value of 0. Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))ĮC50 is the concentration of agonist that gives a response half way between Bottom and Top. This is not the same as the response at Y=50. Depending on which units Y is expressed in, and the values of Bottom and Top, the EC50 may give a response nowhere near "50". HillSlope describes the steepness of the family of curves. A HillSlope of 1.0 is standard, and you should consider constraining the Hill Slope to a constant value of 1.0. #Graphpad prism 4 parameter logistic curve softwareĪ Hill slope greater than 1.0 is steeper, and a Hill slope less than 1.0 is shallower.
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