![]() Enter one data set into column A, and use columns B, C. If you prefer to enter concentrations, rather than the logarithm of concentrations, use Prism to transform the X values to logs.įrom the data table, click Analyze, choose nonlinear regression, choose the panel of equations "Dose-response curves - Stimulation" and then choose the equation " log(Agonist) vs. Ĭonsider constraining the parameter HillSlope to its standard values of 1.0. This is especially useful if you don't have many data points, and therefore cannot fit the slope very well. ![]() If you have subtracted off any basal response, consider constraining Bottom to a constant value of 0. Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))ĮC50 is the concentration of agonist that gives a response half way between Bottom and Top. This is not the same as the response at Y=50. Depending on which units Y is expressed in, and the values of Bottom and Top, the EC50 may give a response nowhere near "50". ![]() ![]() HillSlope describes the steepness of the family of curves. A HillSlope of 1.0 is standard, and you should consider constraining the Hill Slope to a constant value of 1.0. #Graphpad prism 4 parameter logistic curve softwareĪ Hill slope greater than 1.0 is steeper, and a Hill slope less than 1.0 is shallower. ![]()
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